Eukaryotic Translation Initiation Factor 3, Subunit G (EIF3G) (Center) antibody

Antigen: Eukaryotic Translation Initiation Factor 3, Subunit G (EIF3G)

Synonyms: EIF3S4, EIF3-P42, eIF3-p44, eIF3-delta, p44, 44kDa, D0Jmb4, Eif3s4, TU-189B2, eif3s4, MGC56553, zgc:56553, EIF3G, eIF3g, eIF3-S4

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651535

Additional Information

Alternative name: EIF3G

Gene ID: 8666

UniProt: NP_003746.2, O75821

Immunogen: This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the Central region of human EIF3G.

Isotype: Ig

Description: Other names: eIF3 p42, eIF3 p44, Eukaryotic translation initiation factor 3 RNA-binding subunit, eIF-3 RNA-binding subunit, eIF-3-delta, Eukaryotic translation initiation factor 3 subunit 4, Eukaryotic translation initiation factor 3 subunit G, eIF3g,EIF3S4, EIF3G

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-247 amino acids from the Central region of human EIF3G.

Molecular Weight: 35611  DA

Comments:

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

EIF3G Antibody (Center) (ABIN651535) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the EIF3G antibody detected the EIF3G protein (arrow).

EIF3G Antibody (Center) (ABIN651535) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EIF3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

EIF3G Antibody (Center) (ABIN651535) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Masutani, Sonenberg, Yokoyama et al.: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, 2007 (PubMed).

Zhou, Sandercock, Fraser et al.: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, 2008 (PubMed).