SC31B (C-Term) antibody

Antigen: SC31B

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651805

Additional Information

Gene ID: 25956

UniProt: NP_056305.1, Q9NQW1

Immunogen: This SC31B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from the C-terminal region of human SC31B.

Isotype: Ig

Description: Other names: Protein transport protein Sec31B,SEC31B,SEC31L2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SC31B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from the C-terminal region of human SC31B.

Molecular Weight: 128697 DA

Comments:

Background: This gene encodes a protein of unknown function. The protein has moderate similarity to rat VAP1 protein which is an endosomal membrane-associated protein, containing a putative Ca2+/calmodulin-dependent kinase II phosphorylation site.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

SC31B Antibody (C-term) (ABIN651805) western blot analysis in NCI-H460 cell line lysates (15ug/lane). This demonstrates the SC31B antibody detected the SC31B protein (arrow).

SC31B Antibody (C-term) (ABIN651805) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Tang, Zhang, Low et al.: "Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport." in: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13597-604, 2000 (PubMed).

Grupe, Li, Rowland et al.: "A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease." in: American journal of human genetics, Vol. 78, Issue 1, pp. 78-88, 2005 (PubMed).

Stankewich, Stabach, Morrow: "Human Sec31B: a family of new mammalian orthologues of yeast Sec31p that associate with the COPII coat." in: Journal of cell science, Vol. 119, Issue Pt 5, pp. 958-69, 2006 (PubMed).