BHLH3 (N-Term) antibody

Antigen: BHLH3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651934

Additional Information

Gene ID: 79365

UniProt: Q9C0J9, NP_110389

Immunogen: This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human BHLH3.

Isotype: Ig

Description: Other names: Class E basic helix-loop-helix protein 41,BHLHE41,SHARP1, DEC2, BHLHB3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human BHLH3.

Molecular Weight: 50498 DA

Comments:

Background: BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

BHLH3 Antibody (N-term) (ABIN651934) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the BHLH3 antibody detected the BHLH3 protein (arrow).

BHLH3 Antibody (N-term) (ABIN651934) western blot analysis in mouse cerebellum tissue lysates (15ug/lane). This demonstrates the BHLH3 antibody detected BHLH3 protein (arrow).

BHLH3 Antibody (N-term) (ABIN651934) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Grottke, Mantwill, Dietel et al.: "Identification of differentially expressed genes in human melanoma cells with acquired resistance to various antineoplastic drugs." in: International journal of cancer. Journal international du cancer, Vol. 88, Issue 4, pp. 535-46, 2000 (PubMed).

Fujimoto, Shen, Noshiro et al.: "Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins." in: Biochemical and biophysical research communications, Vol. 280, Issue 1, pp. 164-71, 2001 (PubMed).

Garriga-Canut, Roopra, Buckley: "The basic helix-loop-helix protein, sharp-1, represses transcription by a histone deacetylase-dependent and histone deacetylase-independent mechanism." in: The Journal of biological chemistry, Vol. 276, Issue 18, pp. 14821-8, 2001 (PubMed).

Honma, Kawamoto, Takagi et al.: "Dec1 and Dec2 are regulators of the mammalian molecular clock." in: Nature, Vol. 419, Issue 6909, pp. 841-4, 2002 (PubMed).