Aftiphilin (AFTPH) (C-Term) antibody

Antigen: Aftiphilin (AFTPH)

Synonyms: FLJ20080, FLJ23793, MGC33965, Nbla10388, AU015099, AU016543, AU018432, AU019184, AU044244, 9130023F12Rik, RP23-452C23.3, RGD1311920, 9130023F12RIK, MGC151658, fb71f09, wu:fb71f09, CH73-131E21.7, si:ch73-131e21.7, MGC115640

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651958

Additional Information

Alternative name: AFTPH

Gene ID: 54812

UniProt: Q6ULP2, NP_001002243.1

Immunogen: This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 851-879 amino acids from the C-terminal region of human AFTPH.

Isotype: Ig

Description: Other names: Aftiphilin,AFTPH,AFTH

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 851-879 amino acids from the C-terminal region of human AFTPH.

Molecular Weight: 102200 DA

Comments:

Background: AFTPH may play a role in membrane trafficking.

Synonyms: FLJ20080, FLJ23793, MGC33965, Nbla10388, AU015099, AU016543, AU018432, AU019184, AU044244, 9130023F12Rik, RP23-452C23.3, RGD1311920, 9130023F12RIK, MGC151658, fb71f09, wu:fb71f09, CH73-131E21.7, si:ch73-131e21.7, MGC115640

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

AFTPH Antibody (C-term) (ABIN651958) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AFTPH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

AFTPH Antibody (C-term) (ABIN651958) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AFTPH Antibody (C-term) (ABIN651958) western blot analysis in CEM,Jurkat,MCF-7 cell line lysates (35ug/lane). This demonstrates the AFTPH antibody detected the AFTPH protein (arrow).

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Hirst, Borner, Harbour et al.: "The aftiphilin/p200/gamma-synergin complex." in: Molecular biology of the cell, Vol. 16, Issue 5, pp. 2554-65, 2005 (PubMed).

Burman, Wasiak, Ritter et al.: "Aftiphilin is a component of the clathrin machinery in neurons." in: FEBS letters, Vol. 579, Issue 10, pp. 2177-84, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Lui-Roberts, Ferraro, Nightingale et al.: "Aftiphilin and gamma-synergin are required for secretagogue sensitivity of Weibel-Palade bodies in endothelial cells." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5072-81, 2008 (PubMed).