Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3) (Center) antibody

Antigen: Reticulocalbin 3, EF-Hand Calcium Binding Domain (RCN3)

Synonyms: RLP49, MGC102610, D7Ertd671e, 6030455P07Rik, D530026G20Rik

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651973

Additional Information

Alternative name: RCN3

Gene ID: 57333

UniProt: NP_065701.2, Q96D15

Immunogen: This RCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-171 amino acids from the Central region of human RCN3.

Isotype: Ig

Description: Other names: Reticulocalbin-3,RCN3,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-171 amino acids from the Central region of human RCN3.

Molecular Weight: 37493 DA

Comments:

Background: Calcipressin 3 inhibits calcineurin dependent transcriptional responses by binding to the catalytic domain of calcineurin A, and may play a role during central nervous system development. Highest expression occurs in heart, skeletal muscle kidney, liver and peripheral blood leukocytes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

RCN3 Antibody (Center) (ABIN651973) western blot analysis in mouse lung tissue lysates (15ug/lane). This demonstrates the RCN3 antibody detected RCN3 protein (arrow).

RCN3 Antibody (Center) (ABIN651973) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Hu, Han, Song et al.: "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9543-8, 2000 (PubMed).

Tsuji, Kikuchi, Sato et al.: "A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4." in: The Biochemical journal, Vol. 396, Issue 1, pp. 51-9, 2006 (PubMed).