AAK1 (N-Term) antibody

Antigen: AAK1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652404

Additional Information

Gene ID: 22848

UniProt: Q2M2I8

Immunogen: This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~223 amino acids from the n-terminal region of human AAK1.

Isotype: Ig

Description: Other names: AP2-associated protein kinase 1, Adaptor-associated kinase 1, AAK1, KIAA1048

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194~223 amino acids from the n-terminal region of human AAK1.

Molecular Weight: 93578 DA

Comments:

Background: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of AAK1 Antibody (N-term) (ABIN652404) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.

Publications

Product: Takahashi, Furuchi, Naganuma: "Endocytic Ark/Prk kinases play a critical role in adriamycin resistance in both yeast and mammalian cells." in: Cancer research, Vol. 66, Issue 24, pp. 11932-7, 2006 (PubMed).

Henderson, Conner: "A novel AAK1 splice variant functions at multiple steps of the endocytic pathway." in: Molecular biology of the cell, Vol. 18, Issue 7, pp. 2698-706, 2007 (PubMed).