NEK3 (Center) antibody

Antigen: NEK3

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652427

Additional Information

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Nek3, NimA-related protein kinase 3, HSPK 36

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339~368 amino acids from the center region of human NEK3.

Comments:

Background: In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.

Application Details

Concentration: 0.25 mg/ml

Purification: Purified

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

NEK3 Antibody (K346) (ABIN652427) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NEK3 antibody detected the NEK3 protein (arrow).

Western blot analysis of NEK3 (arrow) using rabbit polyclonal NEK3 Antibody (K346) (ABIN652427). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.

Publications

Product: Schultz, Fry, Sütterlin et al.: "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 625-35, 1994 (PubMed).

Schultz, Nigg: "Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 4, Issue 10, pp. 821-30, 1994 (PubMed).

Kimura, Okano: "Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3)." in: Cytogenetics and cell genetics, Vol. 95, Issue 3-4, pp. 177-82, 2002 (PubMed).