phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (Center) antibody

Antigen: phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652449

Additional Information

Alternative name: PLA2G4A

Gene ID: 5321

UniProt: P47712

Immunogen: This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520~549 amino acids from the Center region of human PLA2G4A.

Isotype: Ig

Description: Other names: Cytosolic phospholipase A2, cPLA2, Phospholipase A2 group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4, CPLA2, PLA2G4A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520~549 amino acids from the Center region of human PLA2G4A.

Molecular Weight: 85211 DA

Comments:

Background: PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of PLA2G4A Antibody (Center) (ABIN652449) in NCI-H460 cell line lysates (35ug/lane). PLA2G4A (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Sharp, White, Chiou et al.: "Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2." in: The Journal of biological chemistry, Vol. 266, Issue 23, pp. 14850-3, 1991 (PubMed).

Clark, Lin, Kriz et al.: "A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP." in: Cell, Vol. 65, Issue 6, pp. 1043-51, 1991 (PubMed).