BRISC and BRCA1 A Complex Member 1 (BABAM1) (Center) antibody

Antigen: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Synonyms: NBA1, HSPC142, MERIT40, FLJ20571, Merit40, MGC94542, merit40, MGC84305, nba1, C19orf62, MGC139524, C7H19orf62, DKFZp468C208

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652514

Additional Information

Alternative name: HSPC142

Gene ID: 29086

UniProt: Q9NWV8

Immunogen: This HSPC142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human HSPC142

Isotype: Ig

Description: Other names: BRCA1-A complex subunit MERIT40, Mediator of RAP80 interactions and targeting subunit of 40 kDa, New component of the BRCA1-A complex, HSPC142, NBA1, C19orf62, MERIT40

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HSPC142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human HSPC142

Molecular Weight: 36560 DA

Comments:

Background: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of HSPC142 Antibody (Center) (ABIN652514) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.

Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Shao, Patterson-Fortin, Messick et al.: "MERIT40 controls BRCA1-Rap80 complex integrity and recruitment to DNA double-strand breaks." in: Genes & development, Vol. 23, Issue 6, pp. 740-54, 2009 (PubMed).