Estrogen-Related Receptor alpha (ESRRA) (Center) antibody

Antigen: Estrogen-Related Receptor alpha (ESRRA)

Synonyms: ERR1, ERRa, ESRL1, NR3B1, ERRalpha, Err1, Nr3b1, Estrra, Errra, ESRRA, ESTRRA, err1, erra, esrral, erralpha, nr3b1, MGC80854, esrl1, MGC147343, LOC100125507

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652575

Additional Information

Alternative name: ESRRA

Gene ID: 2101

UniProt: P11474

Immunogen: This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the Center region of human ESRRA.

Isotype: Ig

Description: Other names: Steroid hormone receptor ERR1, Estrogen-related receptor, alpha, ERR-alpha, Estrogen receptor-like 1, Nuclear receptor subfamily 3 group B member 1, NR3B1, ESRL1, ERR1, ESRRA

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ESRRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the Center region of human ESRRA.

Molecular Weight: 45510 DA

Comments:

Background: ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of ESRRA Antibody (Center) (ABIN652575) in A375 cell line lysates (35ug/lane). ESRRA (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with ESRRA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of 293 cells using ESRRA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Wiley, Kraus, Zuo et al.: "SV40 early-to-late switch involves titration of cellular transcriptional repressors." in: Genes & development, Vol. 7, Issue 11, pp. 2206-19, 1993 (PubMed).

Yang, Shigeta, Shi et al.: "Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter." in: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5795-804, 1996 (PubMed).