Centrosomal Protein 128kDa (CEP128) (Center) antibody

Antigen: Centrosomal Protein 128kDa (CEP128)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652929

Additional Information

Alternative name: C14orf145

Gene ID: 145508

UniProt: Q6ZU80

Immunogen: This C14orf145 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 913~943 amino acids from the Center region of human C14orf145 .

Isotype: Ig

Description: Other names: Uncharacterized protein C14orf145, C14orf145, C14orf61

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C14orf145 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 913~943 amino acids from the Center region of human C14orf145 .

Molecular Weight: 128015 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of C14orf145 Antibody (Center) (ABIN652929) in HL-60 cell line lysates (35ug/lane). C14orf145 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis reacted with C14orf145 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

C14orf145 Antibody (Center) (ABIN652929) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).