Denatured ATG1 antibody

Details for Product No. ABIN653062
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Human, Mouse (Murine)
Clonality (Clone)
Polyclonal ()
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
Pubmed 1 reference available
Catalog no. ABIN653062
Quantity 400 µL
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Immunogen This denatured ATG1 antibody is generated from rabbits immunized with a denatured human ATG1 recombinant protein.
Clone RB17499
Isotype Ig
Specificity This Denatured ATG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human ATG1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background ULK1 is involved in autophagy. It is required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. It is involved in axon growth (By similarity) and plays an essential role in neurite extension of cerebellar granule cells (By similarity).
Synonyms: Unc-51-like kinase 1, Serine/threonine-protein kinase ULK1, KIAA0722, ULK1
Molecular Weight 112601 DA
Gene ID 8408
UniProt O75385
Research Area Autophagy
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Denatured ATG1 antibody Western blot analysis of Denatured ATG1 (ABIN653062) in mouse cerebellum tissue lysates (35 µg/lane). ATG1 (arrow) was detected using the purified polyclonal antibody.
anti-Denatured ATG1 antibody (2) Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Denatured ATG1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Denatured ATG1 antibody (3) Denatured ATG1 Antibody(ABIN653062) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Greenman, Stephens, Smith et al.: "Patterns of somatic mutation in human cancer genomes...." in: Nature, Vol. 446, Issue 7132, pp. 153-8, 2007 (PubMed).

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