Denatured ATG1 antibody

Antigen: Denatured ATG1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653062

Additional Information

Gene ID: 8408

UniProt: O75385

Immunogen: This Denatured ATG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human ATG1.

Isotype: Ig

Description: Other names: Unc-51-like kinase 1, Serine/threonine-protein kinase ULK1, KIAA0722, ULK1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Denatured ATG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human ATG1.

Molecular Weight: 112601 DA

Comments:

Background: ULK1 is involved in autophagy. It is required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. It is involved in axon growth (By similarity) and plays an essential role in neurite extension of cerebellar granule cells (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of Denatured ATG1 (ABIN653062) in mouse cerebellum tissue lysates (35ug/lane). ATG1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Denatured ATG1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Denatured ATG1 Antibody(ABIN653062) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Greenman, Stephens, Smith et al.: "Patterns of somatic mutation in human cancer genomes." in: Nature, Vol. 446, Issue 7132, pp. 153-8, 2007 (PubMed).