HRIHFB2025 (C-Term) antibody

Antigen: HRIHFB2025

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653093

Additional Information

Gene ID: 25776

UniProt: Q9Y3M2

Immunogen: This HRIHFB2025 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino acids from the C-terminal region of human HRIHFB2025.

Isotype: Ig

Description: Other names: ARPP-binding protein, Cytosolic leucine-rich protein, PIGEA-14, PKD2 interactor, Golgi and endoplasmic reticulum-associated 1, Protein chibby homolog 1, HRIHFB2025, PGEA1, CBY, C22orf2, ARB1, CBY1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HRIHFB2025 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino acids from the C-terminal region of human HRIHFB2025.

Molecular Weight: 14470 DA

Comments:

Background: Inhibits the Wnt/Wingless pathway by binding to beta catenin and inhibiting beta catenin mediated transcriptional activation by competing with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin 2/PKD2 and possibly of other intracellular proteins.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of HRIHFB2025 Antibody (C-term) (ABIN653093) in MDA-MB231 cell line lysates (35ug/lane). HRIHFB2025 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with HRIHFB2025 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Gauci, Helbig, Slijper et al.: "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." in: Analytical chemistry, Vol. 81, Issue 11, pp. 4493-501, 2009 (PubMed).