WD Repeat Domain 37 (WDR37) (C-Term) antibody

Antigen: WD Repeat Domain 37 (WDR37)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653198

Additional Information

Alternative name: WDR37

Gene ID: 22884

UniProt: Q9Y2I8

Immunogen: This WDR37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433~463 amino acids from the C-terminal region of human WDR37.

Isotype: Ig

Description: Other names: WD repeat-containing protein 37, KIAA0982, WDR37

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WDR37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433~463 amino acids from the C-terminal region of human WDR37.

Molecular Weight: 54665 DA

Comments:

Background: WDR37 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of WDR37 Antibody (C-term) (ABIN653198) in HepG2 cell line lysates (35ug/lane). WDR37 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with WDR37 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

WDR37 Antibody (C-term) (ABIN653198) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).