Lysine (K)-Specific Demethylase 4B (KDM4B) (N-Term) antibody

Antigen: Lysine (K)-Specific Demethylase 4B (KDM4B)

Synonyms: JMJD2B, FLJ44906, KIAA0876, Jmjd2b, MGC6349, mKIAA0876, 4732474L06Rik, KDM4B, jmjd2b, si:ch211-124a3.4

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653341

Additional Information

Alternative name: JMJD2B

Gene ID: 23030

UniProt: O94953

Immunogen: This JMJD2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~40 amino acids from the N-terminal region of human JMJD2B.

Isotype: Ig

Description: Other names: Jumonji domain-containing protein 2B, JmjC domain-containing histone demethylation protein 3B, Lysine-specific demethylase 4B, KIAA0876, JMJD2B, JHDM3B, KDM4B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This JMJD2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~40 amino acids from the N-terminal region of human JMJD2B.

Molecular Weight: 121897 DA

Comments:

Background: KDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of JMJD2B Antibody (N-term) (ABIN653341) in K562 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

JMJD2B Antibody (N-term) (ABIN653341) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Gray, Iglesias, Lizcano et al.: "Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein." in: The Journal of biological chemistry, Vol. 280, Issue 31, pp. 28507-18, 2005 (PubMed).

Beyer, Kristensen, Jensen et al.: "The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF." in: The Journal of biological chemistry, Vol. 283, Issue 52, pp. 36542-52, 2008 (PubMed).