EXT2 (Center) antibody

Antigen: EXT2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653350

Additional Information

Gene ID: 2132

UniProt: Q93063

Immunogen: This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~218 amino acids from the Central region of human EXT2.

Isotype: Ig

Description: Other names: Multiple exostoses protein 2, Putative tumor suppressor protein EXT2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Exostosin-2, EXT2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~218 amino acids from the Central region of human EXT2.

Molecular Weight: 82255 DA

Comments:

Background: EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EXT2 Antibody (Center) (ABIN653350) in MDA-MB468 cell line lysates (35ug/lane). EXT2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

EXT2 Antibody (Center) (ABIN653350) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Zhao, Bradfield, Zhang et al.: "Examination of all type 2 diabetes GWAS loci reveals HHEX-IDE as a locus influencing pediatric BMI." in: Diabetes, Vol. 59, Issue 3, pp. 751-5, 2010 (PubMed).