Diaphanous Homolog 2 (Drosophila) (DIAPH2) (Center) antibody

Antigen: Diaphanous Homolog 2 (Drosophila) (DIAPH2)

Synonyms: DIAPH2, Diap2, RGD1564399, DIAPH1, MGC148327, DIA, POF, DIA2, DRF2, POF2, mDia3, FLJ11167, DIA3, E430022I22Rik, dia2, sb:eu1025

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653356

Additional Information

Alternative name: DIAPH2

Gene ID: 1730

UniProt: O60879

Immunogen: This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 877~907 amino acids from the Central region of human DIAPH2.

Isotype: Ig

Description: Other names: Diaphanous-related formin-2, DRF2, Protein diaphanous homolog 2, DIA, DIAPH2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 877~907 amino acids from the Central region of human DIAPH2.

Molecular Weight: 125569 DA

Comments:

Background: DIAPH2 belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this protein have been linked to premature ovarian failure 2.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DIAPH2 Antibody (Center) (ABIN653356) in 293, MDA-MB435 cell line lysates (35ug/lane). DIAPH2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

DIAPH2 Antibody (Center) (ABIN653356) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Satoh, Tominaga: "mDia-interacting protein acts downstream of Rho-mDia and modifies Src activation and stress fiber formation." in: The Journal of biological chemistry, Vol. 276, Issue 42, pp. 39290-4, 2001 (PubMed).

Gasman, Kalaidzidis, Zerial: "RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase." in: Nature cell biology, Vol. 5, Issue 3, pp. 195-204, 2003 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).