GINS Complex Subunit 1 (Psf1 Homolog) (GINS1) (N-Term) antibody

Antigen: GINS Complex Subunit 1 (Psf1 Homolog) (GINS1)

Synonyms: PSF1, KIAA0186, RP4-691N24.2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653447

Additional Information

Alternative name: GINS1

Gene ID: 9837

UniProt: Q14691

Immunogen: This GINS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human GINS1.

Isotype: Ig

Description: Other names: GINS complex subunit 1, DNA replication complex GINS protein PSF1, PSF1, KIAA0186, GINS1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GINS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human GINS1.

Molecular Weight: 22988 DA

Comments:

Background: The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4, MIM 610611), Psf1, Psf2 (GINS2, MIM 610609), and Psf3 (GINS3, MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GINS1 Antibody (N-term) (ABIN653447) in MCF-7 cell line lysates (35ug/lane). GINS1 (arrow) was detected using the purified Pab.

Publications

Product: Nagahama, Ueno, Miyamoto et al.: "PSF1, a DNA replication factor expressed widely in stem and progenitor cells, drives tumorigenic and metastatic properties." in: Cancer research, Vol. 70, Issue 3, pp. 1215-24, 2010 (PubMed).