G Antigen 7 (GAGE7) (C-Term) antibody

Antigen: G Antigen 7 (GAGE7)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653712

Additional Information

Alternative name: GAGE7

Gene ID: 645073

UniProt: O76087

Immunogen: This GAGE7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~117 amino acids from the C-terminal region of human GAGE7.

Isotype: Ig

Description: Other names: Cancer/testis antigen 4.7, CT4.7, AL4, GAGE-8, GAGE-7B, GAGE-12I, G antigen 7, GAGE-7,GAGE7

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GAGE7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88~117 amino acids from the C-terminal region of human GAGE7.

Molecular Weight: 12978 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of GAGE7 Antibody (C-term) (ABIN653712) in K562 cell line lysates (35ug/lane). GAGE7 (arrow) was detected using the purified Pab.

GAGE7 Antibody (C-term) (ABIN653712) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAGE7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GAGE7 Antibody (C-term) (ABIN653712) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Gjerstorff, Ditzel: "An overview of the GAGE cancer/testis antigen family with the inclusion of newly identified members." in: Tissue antigens, Vol. 71, Issue 3, pp. 187-92, 2008 (PubMed).