Nuclear Prelamin A Recognition Factor-Like (NARFL) (Center) antibody

Antigen: Nuclear Prelamin A Recognition Factor-Like (NARFL)

Synonyms: PRN, HPRN, IOP1, LET1L, FLJ21988, 19.m02295

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653781

Additional Information

Alternative name: NARFL

Gene ID: 64428

UniProt: Q9H6Q4

Immunogen: This NARFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247~277 amino acids from the Central region of human NARFL.

Isotype: Ig

Description: Other names: Iron-only hydrogenase-like protein 1, IOP1, Protein related to Narf, Nuclear prelamin A recognition factor-like protein, Cytosolic Fe-S cluster assembly factor NARFL,PRN, NARFL

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NARFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247~277 amino acids from the Central region of human NARFL.

Molecular Weight: 53020 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of NARFL Antibody (Center) (ABIN653781) in MDA-MB435 cell line lysates (35ug/lane). NARFL (arrow) was detected using the purified Pab.

NARFL Antibody (Center) (ABIN653781) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Huang, Song, Flores et al.: "IOP1, a novel hydrogenase-like protein that modulates hypoxia-inducible factor-1alpha activity." in: The Biochemical journal, Vol. 401, Issue 1, pp. 341-52, 2006 (PubMed).

Song, Tu, Lee: "Human ISCA1 interacts with IOP1/NARFL and functions in both cytosolic and mitochondrial iron-sulfur protein biogenesis." in: The Journal of biological chemistry, Vol. 284, Issue 51, pp. 35297-307, 2009 (PubMed).