K2C78 (Center) antibody

Antigen: K2C78

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653813

Additional Information

Gene ID: 196374

UniProt: Q8N1N4

Immunogen: This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182~212 amino acids from the Central region of human K2C78.

Isotype: Ig

Description: Other names: Keratin-5b, Type-II keratin Kb40, Keratin-78, K78, Cytokeratin-78, CK-78, Keratin, type II cytoskeletal 78, KB40, K5B, KRT78

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182~212 amino acids from the Central region of human K2C78.

Molecular Weight: 56866 DA

Comments:

Background: This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of K2C78 Antibody (Center) (ABIN653813) in Hela cell line lysates (35ug/lane). K2C78 (arrow) was detected using the purified Pab.

K2C78 Antibody (Center) (ABIN653813) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Schweizer, Bowden, Coulombe et al.: "New consensus nomenclature for mammalian keratins." in: The Journal of cell biology, Vol. 174, Issue 2, pp. 169-74, 2006 (PubMed).