DCP1 Decapping Enzyme Homolog B (S. Cerevisiae) (DCP1B) (Center) antibody

Antigen: DCP1 Decapping Enzyme Homolog B (S. Cerevisiae) (DCP1B)

Synonyms: DCP1, hDcp1b, DKFZp459H152

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653844

Additional Information

Alternative name: DCP1B

Gene ID: 196513

UniProt: Q8IZD4

Immunogen: This DCP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268~298 amino acids from the Central region of human DCP1B.

Isotype: Ig

Description: Other names: mRNA-decapping enzyme 1B,DCP1B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DCP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268~298 amino acids from the Central region of human DCP1B.

Molecular Weight: 67851 DA

Comments:

Background: DCP1B is a core component of the mRNA decapping complex, a key factor in the regulation of mRNA decay.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DCP1B Antibody (center) (ABIN653844) in Ramos cell line lysates (35ug/lane). DCP1B (arrow) was detected using the purified Pab.

DCP1B Antibody (center) (ABIN653844) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).