M11D1 (N-Term) antibody

Antigen: M11D1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653932

Additional Information

Gene ID: 64745

UniProt: Q9H7H0

Immunogen: This M11D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 90-120 from the N-terminal region of human M11D1.

Isotype: Ig

Description: Other names: False p73 target gene protein, Methyltransferase 11 domain-containing protein 1, Protein RSM22 homolog, mitochondrial,METT11D1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This M11D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 90-120 from the N-terminal region of human M11D1.

Molecular Weight: 50734 DA

Comments:

Background: M11D1 may be a component of the mitochondrial small ribosomal subunit.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of M11D1 Antibody (N-term) (ABIN653932) in HL-60 cell line lysates (35ug/lane). M11D1 (arrow) was detected using the purified Pab.

M11D1 Antibody (N-term) (ABIN653932) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the M11D1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

M11D1 Antibody (N-term) (ABIN653932) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Saveanu, Fromont-Racine, Harington et al.: "Identification of 12 new yeast mitochondrial ribosomal proteins including 6 that have no prokaryotic homologues." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 15861-7, 2001 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).