Aminoadipate Aminotransferase (AADAT) (Center) antibody

Antigen: Aminoadipate Aminotransferase (AADAT)

Synonyms: KAT2, KATII, Aadt, Kat2, mKat-2, AI875679, MGC80030, AADAT, MGC133944

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654120

Additional Information

Alternative name: AADAT

Gene ID: 51166

UniProt: Q8N5Z0, NP_872603.1, NP_057312.1

Immunogen: This AADAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the Central region of human AADAT.

Isotype: Ig

Description: Other names: Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial,AADAT,KAT2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AADAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-232 amino acids from the Central region of human AADAT.

Molecular Weight: 47352 DA

Comments:

Background: This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

AADAT Antibody (Center) (ABIN654120) western blot analysis in HepG2,Y79 cell line lysates (35ug/lane). This demonstrates the AADAT antibody detected the AADAT protein (arrow).

Publications

Product: Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).

Han, Robinson, Li: "Crystal structure of human kynurenine aminotransferase II." in: The Journal of biological chemistry, Vol. 283, Issue 6, pp. 3567-73, 2008 (PubMed).