ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H) (Center) antibody

Antigen: ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H)

Synonyms: ATPQ, MGC128531, MGC82361

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654144

Additional Information

Alternative name: ATP5H

Gene ID: 10476

UniProt: NP_001003785.1, NP_006347.1, O75947

Immunogen: This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ATP5H.

Isotype: Ig

Description: Other names: ATP synthase subunit d, mitochondrial,ATP5H,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ATP5H.

Molecular Weight: 18491 DA

Comments:

Background: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Oster, Wang: "Rotary protein motors." in: Trends in cell biology, Vol. 13, Issue 3, pp. 114-21, 2003 (PubMed).

Cross: "Molecular motors: turning the ATP motor." in: Nature, Vol. 427, Issue 6973, pp. 407-8, 2004 (PubMed).

Kim, Kim, Yoo et al.: "Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells." in: Cancer science, Vol. 99, Issue 10, pp. 1884-91, 2008 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).