WAP Four-Disulfide Core Domain 12 (WFDC12) (C-Term) antibody

Antigen: WAP Four-Disulfide Core Domain 12 (WFDC12)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654327

Additional Information

Alternative name: WFDC12

Gene ID: 128488

UniProt: Q8WWY7, NP_543145.1

Immunogen: This WFDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the C-terminal region of human WFDC12.

Isotype: Ig

Description: Other names: WAP four-disulfide core domain protein 12,WFDC12,C20orf122, WAP2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WFDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the C-terminal region of human WFDC12.

Molecular Weight: 12050 DA

Comments:

Background: This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

WFDC12 antibody (C-term) (ABIN654327) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WFDC12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Lundwall, Clauss: "Identification of a novel protease inhibitor gene that is highly expressed in the prostate." in: Biochemical and biophysical research communications, Vol. 290, Issue 1, pp. 452-6, 2002 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Clauss, Lilja, Lundwall: "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein." in: The Biochemical journal, Vol. 368, Issue Pt 1, pp. 233-42, 2002 (PubMed).

Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Clauss, Lilja, Lundwall: "The evolution of a genetic locus encoding small serine proteinase inhibitors." in: Biochemical and biophysical research communications, Vol. 333, Issue 2, pp. 383-9, 2005 (PubMed).