Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3) (Center) antibody

Antigen: Guanine Nucleotide Binding Protein, alpha Transducing 3 (GNAT3)

Synonyms: gnat-3, Gtn, Ggust, GNAT3, GDCA

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654441

Additional Information

Alternative name: GNAT3

Gene ID: 346562

UniProt: A8MTJ3, NP_001095856.1

Immunogen: This GNAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of human GNAT3.

Isotype: Ig

Description: Other names: GNAT3,Guanine nucleotide-binding protein G(t) subunit alpha-3,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GNAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the Central region of human GNAT3.

Molecular Weight: 40357 DA

Comments:

Background: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase, Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Fehr, Meyer, Widmayer et al.: "Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa." in: Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, Vol. 193, Issue 1, pp. 21-34, 2007 (PubMed).

Jang, Kokrashvili, Theodorakis et al.: "Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 38, pp. 15069-74, 2007 (PubMed).