Family with Sequence Similarity 20, Member C (FAM20C) (C-Term) antibody

Antigen: Family with Sequence Similarity 20, Member C (FAM20C)

Synonyms: RNS, DMP4, DKFZp547C074

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654936

Additional Information

Alternative name: DMP4

Gene ID: 56975

UniProt: Q8IXL6, NP_064608.2

Immunogen: This DMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-471 amino acids from the C-terminal region of human DMP4.

Isotype: Ig

Description: Other names: Dentin matrix protein 4,FAM20C,DMP4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-471 amino acids from the C-terminal region of human DMP4.

Molecular Weight: 64446 DA

Comments:

Background: Calcium-binding protein which may play a role in dentin mineralization (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

DMP4 antibody (C-term) (ABIN654936) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DMP4 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Hao, Narayanan, Muni et al.: "Dentin matrix protein 4, a novel secretory calcium-binding protein that modulates odontoblast differentiation." in: The Journal of biological chemistry, Vol. 282, Issue 21, pp. 15357-65, 2007 (PubMed).

Simpson, Hsu, Keir et al.: "Mutations in FAM20C are associated with lethal osteosclerotic bone dysplasia (Raine syndrome), highlighting a crucial molecule in bone development." in: American journal of human genetics, Vol. 81, Issue 5, pp. 906-12, 2007 (PubMed).

Simpson, Scheuerle, Hurst et al.: "Mutations in FAM20C also identified in non-lethal osteosclerotic bone dysplasia." in: Clinical genetics, Vol. 75, Issue 3, pp. 271-6, 2009 (PubMed).