APG7L-D555 (PEI 1:100)b antibody

Antigen: APG7L-D555 (PEI 1:100)b

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655142

Additional Information

Gene ID: 10533

UniProt: O95352, NP_006386.1, NP_001129503.2, NP_001138384.1

Immunogen: This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547-576 amino acids from human APG7L.

Isotype: Ig

Description: Other names: Ubiquitin-like modifier-activating enzyme ATG7,ATG7,APG7L

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This APG7L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547-576 amino acids from human APG7L.

Molecular Weight: 77960 DA

Comments:

Background: This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

APG7L Antibody (ABIN655142) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Zhao, Yang, Liao et al.: "Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity." in: Nature cell biology, Vol. 12, Issue 7, pp. 665-75, 2010 (PubMed).

Metzger, Saukko, Van Che et al.: "Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7." in: Human genetics, Vol. 128, Issue 4, pp. 453-9, 2010 (PubMed).