Sp1 Transcription Factor (SP1) (C-Term,Pro692) antibody

Antigen: Sp1 Transcription Factor (SP1)

Synonyms: Sp1-1, AA450830, AI845540, 1110003E12Rik, fc18h09, zgc:85950, wu:fc18h09, MGC76006, MGC83896, SP1

Binding Site: C-Term,Pro692

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655398

Additional Information

Alternative name: SP1

Gene ID: 6667

UniProt: NP_612482.2, NP_003100.1, P08047

Immunogen: This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-715 amino acids from the C-terminal region of human SP1.

Isotype: Ig

Description: Other names: SP1,Transcription factor Sp1,TSFP1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-715 amino acids from the C-terminal region of human SP1.

Molecular Weight: 80693 DA

Comments:

Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Signaling, Protein Modifications, Phospho-specific antibodies, Transcription Factors, Translation Factors, Ubiquitin-related antibodies

Restrictions: For Research Use only

Images

SP1 Antibody (C-term P692) (ABIN655398) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Jutooru, Chadalapaka, Lei et al.: "Inhibition of NFkappaB and pancreatic cancer cell and tumor growth by curcumin is dependent on specificity protein down-regulation." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25332-44, 2010 (PubMed).

Imanishi, Imamura, Higashi et al.: "Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp1." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 625-30, 2010 (PubMed).

Pan, Yang, Wei et al.: "SP1 acts as a key factor, contributes to upregulation of ADAM23 expression under serum deprivation." in: Biochemical and biophysical research communications, Vol. 401, Issue 2, pp. 306-12, 2010 (PubMed).

Mucha, Ooi, Linley et al.: "Transcriptional control of KCNQ channel genes and the regulation of neuronal excitability." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13235-45, 2010 (PubMed).