|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Sp1 Transcription Factor (SP1) (C-Term,Pro692) antibody
|Synonyms||Sp1-1, AA450830, AI845540, 1110003E12Rik, fc18h09, zgc:85950, wu:fc18h09, MGC76006, MGC83896, SP1|
Alternatives Western Blotting (WB), ELISA
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|UniProt||NP_612482.2, NP_003100.1, P08047|
|Immunogen||This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-715 amino acids from the C-terminal region of human SP1.|
|Description||Other names: SP1,Transcription factor Sp1,TSFP1|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-715 amino acids from the C-terminal region of human SP1.|
|Molecular Weight||80693 DA|
Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure, Signaling, Protein Modifications, Phospho-specific antibodies, Transcription Factors, Translation Factors, Ubiquitin-related antibodies|
|Restrictions||For Research Use only|
|SP1 Antibody (C-term P692) (ABIN655398) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.|
Jutooru, Chadalapaka, Lei et al.: "Inhibition of NFkappaB and pancreatic cancer cell and tumor growth by curcumin is dependent on specificity protein down-regulation." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25332-44, 2010 (PubMed).
Imanishi, Imamura, Higashi et al.: "Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Sp1." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 625-30, 2010 (PubMed).
Pan, Yang, Wei et al.: "SP1 acts as a key factor, contributes to upregulation of ADAM23 expression under serum deprivation." in: Biochemical and biophysical research communications, Vol. 401, Issue 2, pp. 306-12, 2010 (PubMed).
Mucha, Ooi, Linley et al.: "Transcriptional control of KCNQ channel genes and the regulation of neuronal excitability." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13235-45, 2010 (PubMed).
|Hosts||Rabbit (42), Mouse (9), Goat (6)|
|Reactivities||Human (46), Mouse (Murine) (13), Rat (Rattus) (12), Cow (Bovine) (4), Chicken (1), Pig (Porcine) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (51), ELISA (27), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunofluorescence (IF) (12), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1)|
|Epitopes||Internal Region (7), pThr739 (5), AA 609-627 (4), C-Term (3), AA 700-740 (1), AA 750-785 (1), Isoform 2 (1), pThr453 (1)|