|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Lin-28 Homolog A (C. Elegans) (LIN28A) antibody
|Synonyms||CSDD1, LIN28, LIN-28, ZCCHC1, FLJ12457, Lin28, Tex17, Lin-28, Gm10299, AL024421, ENSMUSG00000070700, csdd1, lin-28, lin28a, zcchc1, lin28, MGC55584, sb:cb650, zgc:55584, RGD1566408, MGC97527|
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This LIN28A antibody is generated from rabbits immunized with a His fusion protein from human LIN28A.|
|Description||Other names: Protein lin-28 homolog A,LIN28A,CSDD1, LIN28, ZCCHC1|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This LIN28A antibody is generated from rabbits immunized with a His fusion protein from human LIN28A.|
|Molecular Weight||22743 DA|
Background: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure, Signaling, Chromatin|
|Restrictions||For Research Use only|
West, Viswanathan, Yabuuchi et al.: "A role for Lin28 in primordial germ-cell development and germ-cell malignancy." in: Nature, Vol. 460, Issue 7257, pp. 909-13, 2009 (PubMed).
Heo, Joo, Kim et al.: "TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation." in: Cell, Vol. 138, Issue 4, pp. 696-708, 2009 (PubMed).
Iliopoulos, Hirsch, Struhl: "An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation." in: Cell, Vol. 139, Issue 4, pp. 693-706, 2009 (PubMed).
Qiu, Ma, Wang et al.: "Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells." in: Nucleic acids research, Vol. 38, Issue 4, pp. 1240-8, 2010 (PubMed).
Peng, Maihle, Huang: "Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer." in: Oncogene, Vol. 29, Issue 14, pp. 2153-9, 2010 (PubMed).
|Hosts||Rabbit (42), Mouse (12), Goat (1)|
|Reactivities||Human (53), Mouse (Murine) (32), Rat (Rattus) (32), Cow (Bovine) (5), Dog (Canine) (3), Pig (Porcine) (3), Cat (Feline) (1), Chicken (1), Chimpanzee (1), Rabbit (1), Rhesus Monkey (1)|
|Applications||Western Blotting (WB) (38), ELISA (31), Immunofluorescence (IF) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (IHC) (11), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||C-Term (4), AA 100-150 (1), AA 93-209 (1), Center (1), N-Term (1), pSer134 (1), pSer86 (1)|