Killin, p53-Regulated DNA Replication Inhibitor (KLLN) (C-Term) antibody

Antigen: Killin, p53-Regulated DNA Replication Inhibitor (KLLN)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655558

Additional Information

Alternative name: KILLIN

Gene ID: 100144748

UniProt: B2CW77, NP_001119521.1

Immunogen: This KILLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-171 amino acids from the C-terminal region of human KILLIN.

Isotype: Ig

Description: Other names: Killin

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KILLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-171 amino acids from the C-terminal region of human KILLIN.

Molecular Weight: 19958 DA

Comments:

Background: DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating TP53/p53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double-and single-stranded DNA.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure, Signaling, Transcription Factors

Restrictions: For Research Use only

Images

KILLIN Antibody (C-term) (ABIN655558) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the KILLIN antibody detected the KILLIN protein (arrow).

Publications

Product: Cho, Liang: "Killin is a p53-regulated nuclear inhibitor of DNA synthesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5396-401, 2008 (PubMed).