Acid Phosphatase, Prostate (ACPP) (Center) antibody

Antigen: Acid Phosphatase, Prostate (ACPP)

Synonyms: PAP, ACP3, ACP-3, Lap, Ppal, AI324033, A030005E02Rik, pap, Acpp11, RNACPP11, ACPP, MGC159906, acpt, MGC130636

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655637

Additional Information

Alternative name: PAP

Gene ID: 10914

UniProt: P51003, NP_116021.2

Immunogen: This PAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the Central region of human PAP.

Isotype: Ig

Description: Other names: RB19099

Characteristics: Synthetic peptide

Specificity: This PAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the Central region of human PAP.

Molecular Weight: 82843 DA

Comments:

Background: Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

PAP Antibody (Center) (ABIN655637) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Dettwiler, Aringhieri, Cardinale et al.: "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 35788-97, 2004 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).