Slingshot Homolog 3 (Drosophila) (SSH3) (C-Term) antibody

Antigen: Slingshot Homolog 3 (Drosophila) (SSH3)

Synonyms: SSH3L, FLJ10928, FLJ20515, FLJ42240, SSH-3, mSSH-3L, BC028922, SSH3, MGC143164

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655643

Additional Information

Alternative name: SSH3

Gene ID: 54961

UniProt: NP_060327.3, Q8TE77

Immunogen: This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.

Isotype: Ig

Description: Other names: RB19876

Characteristics: Synthetic peptide

Specificity: This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 582-610 amino acids from the C-terminal region of human SSH3.

Molecular Weight: 72996 DA

Comments:

Background: The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1, MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Kligys, Claiborne, DeBiase et al.: "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes." in: The Journal of biological chemistry, Vol. 282, Issue 44, pp. 32520-8, 2007 (PubMed).