Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1) (Center) antibody

Antigen: Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix Protein (ID1)

Synonyms: ID, bHLHb24, ID1, Idb1, AI323524, D2Wsu140e, ID125A, MGC156482, id6, cb108, wu:fb57e11

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655663

Additional Information

Alternative name: ID1

Gene ID: 3397

UniProt: NP_851998.1, NP_002156.2, P41134

Immunogen: This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of human ID1.

Isotype: Ig

Description: Other names: RB29238

Characteristics: Synthetic peptide

Specificity: This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of human ID1.

Molecular Weight: 16133 DA

Comments:

Background: The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Confocal immunofluorescent analysis of ID1 Antibody (Center)(ABIN655663) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Publications

Product: Qian, Lee, Park et al.: "Id1 enhances RING1b E3 ubiquitin ligase activity through the Mel-18/Bmi-1 polycomb group complex." in: Oncogene, Vol. 29, Issue 43, pp. 5818-27, 2010 (PubMed).

Hamajima, Komori, Preciado et al.: "The role of inhibitor of DNA-binding (Id1) in hyperproliferation of keratinocytes: the pathological basis for middle ear cholesteatoma from chronic otitis media." in: Cell proliferation, Vol. 43, Issue 5, pp. 457-63, 2010 (PubMed).