Eukaryotic Translation Initiation Factor 5A-Like 1 (EIF5AL1) (C-Term) antibody

Antigen: Eukaryotic Translation Initiation Factor 5A-Like 1 (EIF5AL1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655925

Additional Information

Alternative name: EIF5AL1

Gene ID: 143244

UniProt: NP_001093162.1, Q6IS14

Immunogen: This EIF5AL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-130 amino acids from the C-terminal region of human EIF5AL1.

Isotype: Ig

Description: Other names: EIF5AL1,Eukaryotic translation initiation factor 5A-1-like,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EIF5AL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-130 amino acids from the C-terminal region of human EIF5AL1.

Molecular Weight: 16773 DA

Comments:

Background: EIF5AL1is a mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Cell Structure, Protein Modifications, Chromatin

Restrictions: For Research Use only

Images

EIF5AL1 Antibody (C-term) (ABIN655925) western blot analysis in Ramos,A2058 cell line lysates (35ug/lane). This demonstrates the EIF5AL1 antibody detected the EIF5AL1 protein (arrow).

Publications

Product: Koettnitz, Wöhl, Kappel et al.: "Identification of a new member of the human eIF-5A gene family." in: Gene, Vol. 159, Issue 2, pp. 283-4, 1995 (PubMed).

Kim, Sprung, Chen et al.: "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." in: Molecular cell, Vol. 23, Issue 4, pp. 607-18, 2006 (PubMed).