RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2) (N-Term) antibody

Antigen: RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2)

Synonyms: CDC25L, CALDAG-GEFI, rasgrp2-a, MGC52798, xrasgrp2, MGC132357, RASGRP2

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656117

Additional Information

Alternative name: RASGRP2

Gene ID: 10235

UniProt: NP_001092141.1, NP_722541.1, Q7LDG7, NP_001092140.1

Immunogen: This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RASGRP2.

Isotype: Ig

Description: Other names: RASGRP2,RAS guanyl-releasing protein 2,CDC25L, MCG7

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RASGRP2.

Molecular Weight: 75kd Isoform 2. 69248 DA

Comments:

Background: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure, Neurology

Restrictions: For Research Use only

Images

RASGRP2 Antibody (N-term) (ABIN656117) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Eto, Murphy, Kerrigan et al.: "Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 12819-24, 2002 (PubMed).