|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (Center) antibody
|Synonyms||atf-4, MGC68539, zgc:85951, wu:fj01c08, wu:fk30b04, creb2, txreb, creb-2, MGC69565, taxreb67, ATF4, ATF4C, MGC126973, MGC179261, sb:eu681, wu:fb08f07, zgc:171702, CREB2|
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human ATF4.|
|Description||Other names: ATF4,Cyclic AMP-dependent transcription factor ATF-4,CREB2, TXREB|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human ATF4.|
|Molecular Weight||38590 DA|
Background: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||ATF4 Antibody (Center) can be refrigerated at 2-8°C for up to 6 months. For long term storage, place the at -20°C.|
|Research Area||Translation Factors, Phospho-specific antibodies, Cell Structure, Protein Modifications, Transcription Factors, Signaling|
|Restrictions||For Research Use only|
|ATF4 Antibody (Center) (ABIN656176) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ATF4 antibody detected the ATF4 protein (arrow).|
Rzymski, Milani, Pike et al.: "Regulation of autophagy by ATF4 in response to severe hypoxia." in: Oncogene, Vol. 29, Issue 31, pp. 4424-35, 2010 (PubMed).
Wek, Staschke: "How do tumours adapt to nutrient stress?" in: The EMBO journal, Vol. 29, Issue 12, pp. 1946-7, 2010 (PubMed).
Liew, Bochenski, Kawamori et al.: "The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2876-88, 2010 (PubMed).
Frank, Ge, Xie et al.: "Control of activating transcription factor 4 (ATF4) persistence by multisite phosphorylation impacts cell cycle progression and neurogenesis." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33324-37, 2010 (PubMed).
Dey, Baird, Zhou et al.: "Both transcriptional regulation and translational control of ATF4 are central to the integrated stress response." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33165-74, 2010 (PubMed).