Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4) (Center) antibody

Antigen: Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4)

Synonyms: atf-4, MGC68539, zgc:85951, wu:fj01c08, wu:fk30b04, creb2, txreb, creb-2, MGC69565, taxreb67, ATF4, ATF4C, MGC126973, MGC179261, sb:eu681, wu:fb08f07, zgc:171702, CREB2

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656176

Additional Information

Alternative name: ATF4

Gene ID: 468

UniProt: P18848, NP_001666.2

Immunogen: This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human ATF4.

Isotype: Ig

Description: Other names: ATF4,Cyclic AMP-dependent transcription factor ATF-4,CREB2, TXREB

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human ATF4.

Molecular Weight: 38590 DA

Comments:

Background: This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: ATF4 Antibody (Center) can be refrigerated at 2-8°C for up to 6 months. For long term storage, place the at -20°C.

Research Area: Translation Factors, Phospho-specific antibodies, Cell Structure, Protein Modifications, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

ATF4 Antibody (Center) (ABIN656176) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the ATF4 antibody detected the ATF4 protein (arrow).

Publications

Product: Rzymski, Milani, Pike et al.: "Regulation of autophagy by ATF4 in response to severe hypoxia." in: Oncogene, Vol. 29, Issue 31, pp. 4424-35, 2010 (PubMed).

Wek, Staschke: "How do tumours adapt to nutrient stress?" in: The EMBO journal, Vol. 29, Issue 12, pp. 1946-7, 2010 (PubMed).

Liew, Bochenski, Kawamori et al.: "The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells." in: The Journal of clinical investigation, Vol. 120, Issue 8, pp. 2876-88, 2010 (PubMed).

Frank, Ge, Xie et al.: "Control of activating transcription factor 4 (ATF4) persistence by multisite phosphorylation impacts cell cycle progression and neurogenesis." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33324-37, 2010 (PubMed).

Dey, Baird, Zhou et al.: "Both transcriptional regulation and translational control of ATF4 are central to the integrated stress response." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33165-74, 2010 (PubMed).