Spondin 1 (SPON1) (N-Term) antibody

Antigen: Spondin 1 (SPON1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656347

Additional Information

Alternative name: VSGP/F-spondin

Gene ID: 10418

UniProt: Q9HCB6, NP_006099.2

Immunogen: This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human VSGP/F-spondin.

Isotype: Ig

Description: Other names: SPON1,Spondin-1,KIAA0762, VSGP

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-44 amino acids from the N-terminal region of human VSGP/F-spondin.

Molecular Weight: 90973 DA

Comments:

Background: Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: VSGP/F-spondin Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

VSGP/F-spondin Antibody (N-term) (ABIN656347) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VSGP/F-spondin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Pyle-Chenault, Stolk, Molesh et al.: "VSGP/F-spondin: a new ovarian cancer marker." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 26, Issue 5, pp. 245-57, 2005 (PubMed).

Kitagawa, Kudo, Iizuka et al.: "Effect of F-spondin on cementoblastic differentiation of human periodontal ligament cells." in: Biochemical and biophysical research communications, Vol. 349, Issue 3, pp. 1050-6, 2006 (PubMed).

Tan, Duquette, Liu et al.: "The crystal structure of the heparin-binding reelin-N domain of f-spondin." in: Journal of molecular biology, Vol. 381, Issue 5, pp. 1213-23, 2008 (PubMed).