WD Repeat Domain 3 (WDR3) (N-Term) antibody

Antigen: WD Repeat Domain 3 (WDR3)

Synonyms: FLJ12796, AW546279, D030020G18Rik, sb:cb42, wu:fb92b07, MGC76073

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656456

Additional Information

Alternative name: WDR3

Gene ID: 10885

UniProt: NP_006775.1, Q9UNX4

Immunogen: This WDR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-236 amino acids from the N-terminal region of human WDR3.

Isotype: Ig

Description: Other names: WD repeat-containing protein 3,WDR3,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WDR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-236 amino acids from the N-terminal region of human WDR3.

Molecular Weight: 106099 DA

Comments:

Background: This gene encodes a nuclear protein containing 10 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, which usually include a trp-asp at the C-terminal end. Proteins belonging to the WD repeat family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: WDR3 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

WDR3 Antibody (N-term) (ABIN656456) western blot analysis in Hela,K562 cell line lysates (35ug/lane). This demonstrates the WDR3 antibody detected the WDR3 protein (arrow).

Publications

Product: Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

McMahon, Ayllón, Panov et al.: "Ribosomal 18 S RNA processing by the IGF-I-responsive WDR3 protein is integrated with p53 function in cancer cell proliferation." in: The Journal of biological chemistry, Vol. 285, Issue 24, pp. 18309-18, 2010 (PubMed).