Eukaryotic Translation Initiation Factor 3, Subunit C-Like (EIF3CL) (N-Term) antibody

Antigen: Eukaryotic Translation Initiation Factor 3, Subunit C-Like (EIF3CL)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656540

Additional Information

Alternative name: EIF3CL

Gene ID: 8663

UniProt: NP_001032897.1, NP_003743.1, Q99613

Immunogen: This EIF3CL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human EIF3CL.

Isotype: Ig

Description: Other names: Eukaryotic translation initiation factor 3 subunit C,EIF3C,EIF3S8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EIF3CL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human EIF3CL.

Molecular Weight: 105344 DA

Comments:

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: EIF3CL Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

EIF3CL Antibody (N-term) (ABIN656540) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3CL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Masutani, Sonenberg, Yokoyama et al.: "Reconstitution reveals the functional core of mammalian eIF3." in: The EMBO journal, Vol. 26, Issue 14, pp. 3373-83, 2007 (PubMed).

Zhou, Sandercock, Fraser et al.: "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18139-44, 2008 (PubMed).

Imielinski, Baldassano, Griffiths et al.: "Common variants at five new loci associated with early-onset inflammatory bowel disease." in: Nature genetics, Vol. 41, Issue 12, pp. 1335-40, 2009 (PubMed).