Iron-Responsive Element Binding Protein 2 (IREB2) (Center) antibody

Antigen: Iron-Responsive Element Binding Protein 2 (IREB2)

Synonyms: ACO3, IRP2, IRP2AD, FLJ23381, IREB2, im:7153062, DKFZp468N047

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656586

Additional Information

Alternative name: IREB2

Gene ID: 3658

UniProt: NP_004127.1, P48200

Immunogen: This IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-238 amino acids from the Central region of human IREB2.

Isotype: Ig

Description: Other names: Iron-responsive element-binding protein 2,IREB2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-238 amino acids from the Central region of human IREB2.

Molecular Weight: 105045 DA

Comments:

Background: IREB2 is a RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: IREB2 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

IREB2 Antibody (Center) (ABIN656586) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Salahudeen, Thompson, Ruiz et al.: "An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 722-6, 2009 (PubMed).

DeMeo, Mariani, Bhattacharya et al.: "Integration of genomic and genetic approaches implicates IREB2 as a COPD susceptibility gene." in: American journal of human genetics, Vol. 85, Issue 4, pp. 493-502, 2009 (PubMed).

Cho, Boutaoui, Klanderman et al.: "Variants in FAM13A are associated with chronic obstructive pulmonary disease." in: Nature genetics, Vol. 42, Issue 3, pp. 200-2, 2010 (PubMed).

Hansen, Xiao, Rice et al.: "Fine mapping of chromosome 15q25.1 lung cancer susceptibility in African-Americans." in: Human molecular genetics, Vol. 19, Issue 18, pp. 3652-61, 2010 (PubMed).

Ucisik-Akkaya, Davis, Do et al.: "Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection." in: Molecular human reproduction, Vol. 16, Issue 10, pp. 770-7, 2010 (PubMed).