Histone Cluster 1, H2ab (HIST1H2AB) (N-Term) antibody

Antigen: Histone Cluster 1, H2ab (HIST1H2AB)

Synonyms: H2A.1, H2a-53

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656986

Additional Information

Alternative name: HIST1H2AB

Gene ID: 8335, 3012

UniProt: P04908, NP_066390.1, NP_003504.2

Immunogen: This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human HIST1H2AB.

Isotype: Ig

Description: Other names: Histone H2A type 1-B/E,HIST1H2AB,H2AFM

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human HIST1H2AB.

Molecular Weight: 14135 DA

Comments:

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: HIST1H2AB Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

HIST1H2AB Antibody (N-term) (ABIN656986) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Hagiwara, Hidaka, Yamada: "Deimination of histone H2A and H4 at arginine 3 in HL-60 granulocytes." in: Biochemistry, Vol. 44, Issue 15, pp. 5827-34, 2005 (PubMed).

Cao, Tsukada, Zhang: "Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing." in: Molecular cell, Vol. 20, Issue 6, pp. 845-54, 2005 (PubMed).