PHD Finger Protein 17 (PHF17) (N-Term) antibody

Antigen: PHD Finger Protein 17 (PHF17)

Synonyms: JADE1, FLJ22479, KIAA1807, Jade1, AU041499, mKIAA1807, D530048A03Rik

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657355

Additional Information

Alternative name: PHF17

Gene ID: 79960

UniProt: NP_079176.2, Q6IE81, NP_955352.1

Immunogen: This PHF17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-51 amino acids from the N-terminal region of human PHF17.

Isotype: Ig

Description: Other names: Protein Jade-1,PHF17,JADE1, KIAA1807

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PHF17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-51 amino acids from the N-terminal region of human PHF17.

Molecular Weight: 95533 DA

Comments:

Background: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: PHF17 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Apoptosis/Necrosis, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

PHF17 Antibody (N-term) (ABIN657355) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the PHF17 antibody detected the PHF17 protein (arrow).

Publications

Product: Doyon, Cayrou, Ullah et al.: "ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation." in: Molecular cell, Vol. 21, Issue 1, pp. 51-64, 2006 (PubMed).

Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Foy, Song, Chitalia et al.: "Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 43, pp. 28817-26, 2008 (PubMed).

Chitalia, Foy, Bachschmid et al.: "Jade-1 inhibits Wnt signalling by ubiquitylating beta-catenin and mediates Wnt pathway inhibition by pVHL." in: Nature cell biology, Vol. 10, Issue 10, pp. 1208-16, 2008 (PubMed).