Nedd4 Family Interacting Protein 1 (NDFIP1) (Center) antibody

Antigen: Nedd4 Family Interacting Protein 1 (NDFIP1)

Synonyms: N4WBP5, MGC10924, N4wbp5, 0610010M22Rik, zgc:55546, wu:fc17e11, n4wbp5, MGC108163, ndfip1, NDFIP1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657372

Additional Information

Alternative name: NDFIP1

Gene ID: 80762

UniProt: Q9BT67, NP_085048.1

Immunogen: This NDFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-106 amino acids from the Central region of human NDFIP1.

Isotype: Ig

Description: Other names: NEDD4 family-interacting protein 1,NDFIP1,N4WBP5

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NDFIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-106 amino acids from the Central region of human NDFIP1.

Molecular Weight: 24899 DA

Comments:

Background: The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: NDFIP1 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Cell Structure, Protein Modifications, Chromatin, Signaling

Restrictions: For Research Use only

Images

NDFIP1 Antibody (Center) (ABIN657372) western blot analysis in human normal Uterus cell line lysates (35ug/lane). This demonstrates the NDFIP1 antibody detected the NDFIP1 protein (arrow).

Publications

Product: Rapley, Turnbull, Al Olama et al.: "A genome-wide association study of testicular germ cell tumor." in: Nature genetics, Vol. 41, Issue 7, pp. 807-10, 2009 (PubMed).

Howitt, Putz, Lackovic et al.: "Divalent metal transporter 1 (DMT1) regulation by Ndfip1 prevents metal toxicity in human neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 36, pp. 15489-94, 2009 (PubMed).

Mund, Pelham: "Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11429-34, 2010 (PubMed).