Dynactin 4 (p62) (DCTN4) (N-Term) antibody

Antigen: Dynactin 4 (p62) (DCTN4)

Synonyms: C78178, 4930427E12Rik, MGC114292, DCTN4, MGC84654, dctn4, zgc:112431, MGC97564, DKFZp459I0425

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657377

Additional Information

Alternative name: DCTN4

Gene ID: 51164

UniProt: NP_057305.1, Q9UJW0

Immunogen: This DCTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DCTN4.

Isotype: Ig

Description: Other names: Dynactin subunit 4,DCTN4,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DCTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DCTN4.

Molecular Weight: 52337 DA

Comments:

Background: DCTN4 could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: DCTN4 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

DCTN4 Antibody (N-term) (ABIN657377) western blot analysis in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the DCTN4 antibody detected the DCTN4 protein (arrow).

Publications

Product: Karki, Tokito, Holzbaur: "A dynactin subunit with a highly conserved cysteine-rich motif interacts directly with Arp1." in: The Journal of biological chemistry, Vol. 275, Issue 7, pp. 4834-9, 2000 (PubMed).

Boultwood, Fidler, Strickson et al.: "Transcription mapping of the 5q- syndrome critical region: cloning of two novel genes and sequencing, expression, and mapping of a further six novel cDNAs." in: Genomics, Vol. 66, Issue 1, pp. 26-34, 2000 (PubMed).

Ayalon, Davis, Scotland et al.: "An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan." in: Cell, Vol. 135, Issue 7, pp. 1189-200, 2008 (PubMed).