Centromere Protein A (CENPA) (Center) antibody

Antigen: Centromere Protein A (CENPA)

Synonyms: CENP-A, Cenp-A, BcDNA:RE21270, cenH3, CenH3, CenH3[Cid], CENP-A/Cid, CENP-A/CID, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID, CID/CENP-A, Cid[Mel], DmelCG13329, cenpx, cenp-a, sim2, cnp1, Cenp-a, RGD1563607, cenpa, MGC85062

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657764

Additional Information

Alternative name: CENPA

Gene ID: 1058

UniProt: NP_001800.1, P49450, NP_001035891.1

Immunogen: This CENPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human CENPA.

Isotype: Ig

Description: Other names: Histone H3-like centromeric protein A,CENPA,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CENPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human CENPA.

Molecular Weight: 15991 DA

Comments:

Background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].

Synonyms: CENP-A, Cenp-A, BcDNA:RE21270, cenH3, CenH3, CenH3[Cid], CENP-A/Cid, CENP-A/CID, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID, CID/CENP-A, Cid[Mel], DmelCG13329, cenpx, cenp-a, sim2, cnp1, Cenp-a, RGD1563607, cenpa, MGC85062

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: CENPA Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

CENPA Antibody (Center) (ABIN657764) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the CENPA antibody detected the CENPA protein (arrow).

Publications

Product: Carroll, Milks, Straight: "Dual recognition of CENP-A nucleosomes is required for centromere assembly." in: The Journal of cell biology, Vol. 189, Issue 7, pp. 1143-55, 2010 (PubMed).

Sekulic, Bassett, Rogers et al.: "The structure of (CENP-A-H4)(2) reveals physical features that mark centromeres." in: Nature, Vol. 467, Issue 7313, pp. 347-51, 2010 (PubMed).