CUGBP, Elav-Like Family Member 1 (CELF1) (N-Term) antibody

Antigen: CUGBP, Elav-Like Family Member 1 (CELF1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657957

Additional Information

Alternative name: CUGBP1

Gene ID: 10658

UniProt: NP_001166110.1, NP_001020767.1, NP_941989.1, NP_001166111.1

Immunogen: This CUGBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CUGBP1.

Isotype: Ig

Description: Other names: CUGBP Elav-like family member 1,10658,BRUNOL2, CUGBP, CUGBP1, NAB50

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CUGBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CUGBP1.

Molecular Weight: 52063 DA

Comments:

Background: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: CUGBP1 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

CUGBP1 Antibody (N-term) (ABIN657957) western blot analysis in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the CUGBP1 antibody detected the CUGBP1 protein (arrow).

Publications

Product: Tsuda, Kuwasako, Takahashi et al.: "Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3." in: Nucleic acids research, Vol. 37, Issue 15, pp. 5151-66, 2009 (PubMed).

Koshelev, Sarma, Price et al.: "Heart-specific overexpression of CUGBP1 reproduces functional and molecular abnormalities of myotonic dystrophy type 1." in: Human molecular genetics, Vol. 19, Issue 6, pp. 1066-75, 2010 (PubMed).

Le Tonquèze, Gschloessl, Namanda-Vanderbeken et al.: "Chromosome wide analysis of CUGBP1 binding sites identifies the tetraspanin CD9 mRNA as a target for CUGBP1-mediated down-regulation." in: Biochemical and biophysical research communications, Vol. 394, Issue 4, pp. 884-9, 2010 (PubMed).