Barrier To Autointegration Factor 1 (BANF1) (Center) antibody

Antigen: Barrier To Autointegration Factor 1 (BANF1)

Synonyms: BAF, BCRP1, D14S1460, MGC111161, Baf, Bcrp1, L2bp1, L2bp1/Baf, MGC105365, BANF1, DKFZp468C097, C78287, baf, banf1, fa01c01, wu:fa01c01

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657992

Additional Information

Alternative name: BANF1

Gene ID: 8815

UniProt: NP_001137457.1, NP_003851.1, O75531

Immunogen: This BANF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the Central region of human BANF1.

Isotype: Ig

Description: Other names: Barrier-to-autointegration factor,8815,BAF, BCRG1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BANF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the Central region of human BANF1.

Molecular Weight: 10059 DA

Comments:

Background: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: BANF1 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

BANF1 Antibody (Center) (ABIN657992) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the BANF1 antibody detected the BANF1 protein (arrow).

Publications

Product: Haraguchi, Koujin, Osakada et al.: "Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells." in: Journal of cell science, Vol. 120, Issue Pt 12, pp. 1967-77, 2007 (PubMed).

Haraguchi, Kojidani, Koujin et al.: "Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly." in: Journal of cell science, Vol. 121, Issue Pt 15, pp. 2540-54, 2008 (PubMed).

Montes de Oca, Shoemaker, Gucek et al.: "Barrier-to-autointegration factor proteome reveals chromatin-regulatory partners." in: PLoS ONE, Vol. 4, Issue 9, pp. e7050, 2009 (PubMed).