LIM Domain Only 2 (Rhombotin-Like 1) (LMO2) (N-Term) antibody

Antigen: LIM Domain Only 2 (Rhombotin-Like 1) (LMO2)

Synonyms: TTG2, RBTN2, RHOM2, RBTNL1, wu:fc83a08, zgc:111930, MGC124678, MGC139114

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN658023

Additional Information

Alternative name: LMO2

Gene ID: 4005

UniProt: NP_001135788.1, P25791, NP_001135787.1, NP_005565.2

Immunogen: This LMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LMO2.

Isotype: Ig

Description: Other names: Rhombotin-2,LMO2,RBTN2, RBTNL1, RHOM2, TTG2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LMO2.

Molecular Weight: 18358 DA

Comments:

Background: LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: LMO2 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cancer, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

LMO2 Antibody (N-term) (ABIN658023) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the LMO2 antibody detected the LMO2 protein (arrow).

Publications

Product: Hirose, Inukai, Kikuchi et al.: "Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19)." in: Blood, Vol. 116, Issue 6, pp. 962-70, 2010 (PubMed).

Durnick, Law, Maurer et al.: "Expression of LMO2 is associated with t(14;18)/IGH-BCL2 fusion but not BCL6 translocations in diffuse large B-cell lymphoma." in: American journal of clinical pathology, Vol. 134, Issue 2, pp. 278-81, 2010 (PubMed).

Oram, Thoms, Pridans et al.: "A previously unrecognized promoter of LMO2 forms part of a transcriptional regulatory circuit mediating LMO2 expression in a subset of T-acute lymphoblastic leukaemia patients." in: Oncogene, Vol. 29, Issue 43, pp. 5796-808, 2010 (PubMed).

Younes, Beck, Lossos et al.: "Immunoarchitectural patterns in follicular lymphoma: efficacy of HGAL and LMO2 in the detection of the interfollicular and diffuse components." in: The American journal of surgical pathology, Vol. 34, Issue 9, pp. 1266-76, 2010 (PubMed).